No. The liners should never see the inside of a washing machine. Fill a big bucket with a room temp water and dissolve half a cup of liquid laundry detergent in it (no bleach, no fabric softener). You can also use an enzymatic bacterial cleaner but detergent will do just as good a job. Soak the liners for a couple of hours and then rinse them well in cold water and air dry. Oh and take the insoles out before you do it.